Biochemical profiles of rat primary cultured hepatocytes following treatment with rotenone, FCCP, or (+)-usnic acid
نویسندگان
چکیده
منابع مشابه
Variability of DNA Microarray Gene Expression Profiles in Cultured Rat Primary Hepatocytes
DNA microarray is a powerful tool in biomedical research. However, transcriptomic profiling using DNA microarray is subject to many variations including biological variability. To evaluate the different sources of variation in mRNA gene expression profiles, gene expression profiles were monitored using the Affymetrix RatTox U34 arrays in cultured primary hepatocytes derived from six rats over a...
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The mechanism of iron uptake in isolated hepatocytes of Wistar strain female rats was investigated by primary monolayer culture for 24h at 37•Ž. The results were as follows: 1) Iron uptake from diferric transferrin (diFeTf) showed different processes dependent on the ligand concentrations. Namely, the receptor-mediated transport was observed in a lower concentration of diFeTf from 0 to 0.2ƒÊmol...
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Microcystins, which are cyclic heptapeptides produced by some cyanobacterial species from algal blooms, strongly inhibit serine/threonine protein phosphatase and are known as hepatotoxins. Microcystins have many structural variations, yet insufficient information is available on the differences in the cytotoxic potentials among the structural variants. In this study, the cytotoxicities of 16 mi...
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In order to study the effects of saturated fatty acids on ∆6-desaturase activity, rat hepatocytes in primary culture were incubated with lauric (C12:0), myristic (C14:0) or palmitic (C16:0) acids. After optimization, the standard in vitro conditions for the measurement of ∆6desaturase activity were as follows: 60 μmol·L–1 α-linolenic acid (C18:3n-3), reaction time of 20 min and protein content ...
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ژورنال
عنوان ژورنال: The Journal of Toxicological Sciences
سال: 2020
ISSN: 0388-1350,1880-3989
DOI: 10.2131/jts.45.339